Journal: JID Innovations
Article Title: Development of a noninvasive device and method for measuring immune parameters in skin disease
doi: 10.1016/j.xjidi.2026.100456
Figure Lengend Snippet: Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a ) IL-1β, ( b ) TNFα, ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.
Article Snippet: E coli –derived recombinant human TNFα, IL-1β, IL-6, IL-8, IL-10, and IL-18 (endotoxin levels <0.10 EU/μg by the limulus amebocyte lysate method) were purchased from R&D Systems and reconstituted in PBS.
Techniques: Luminex, Control